WebFeb 10, 2024 · Rag1 and Rag2 are expressed exclusively in lymphocytes and are essential in the development and maturation of T and B cells, key components of the adaptive immune system. In the absence of Rag1 or Rag2, lymphocyte development is arrested resulting in T- and B-cell deficiency. WebRAG1 and RAG2 are the key components of the V(D)J recombinase machinery that catalyses the somatic gene rearrangements of antigen receptor genes during …
RAG1 and RAG2 in V(D)J recombination and transposition
WebRAG1 and RAG2 are the key components of the V (D)J recombinase machinery that catalyses the somatic gene rearrangements of antigen receptor genesduring lymphocyte development. In the first step of V (D)J recombination—DNA cleavage—the RAG proteins act together as an endonuclease to excise the DNA between two individual gene segments. WebJul 12, 2024 · Recombination activating genes 1 (Rag1) and Rag2 are expressed in immature lymphocytes and essential for generating the vast repertoire of antigen receptors. Yet, the mechanisms governing the transcription of Rag1 and Rag2 remain to be fully determined, particularly in thymocytes. Combining cDNA microarray and ChIP-seq analysis, we identify … position_jitternudge
Lessons Learned From RAG-1-Deficient Mice in Hypertension
WebJul 21, 2009 · Although it is well established that RAG-1–RAG-2 can function as a recombinase, several studies have shown that RAG-1–RAG-2 is actually a surprisingly … WebThe fractions containing the RAG proteins were collected and dialyzed against 1 L of buffer Q (20 mM Tris-HCl at pH 7.4, 100 mM NaCl, 3 mM MgCl 2, 10% glycerol, and 2 mM DTT) at 4°C overnight. Proteins were loaded on a 1-mL MonoQ column (Pharmacia) and eluted with a 20-mL gradient of NaCl (from 100 to 600 mM). RAG-1 and RAG-2 are proteins at the ends of VDJ genes that separate, shuffle, and rejoin the VDJ genes. This shuffling takes place inside B cells and T cells during their maturation. RAG enzymes work as a multi-subunit complex to induce cleavage of a single double stranded DNA (dsDNA) molecule between the … See more The recombination-activating genes (RAGs) encode parts of a protein complex that plays important roles in the rearrangement and recombination of the genes encoding immunoglobulin and T cell receptor molecules. … See more Based on core sequence homology, it is believed that RAG1 evolved from a transposase from the Transib superfamily. No Transib family members include an N-terminal sequence found in RAG1 suggesting the N-terminal of RAG1 came from a separate … See more • Omenn syndrome • Severe combined immunodeficiency See more In the vertebrate immune system, each antibody is customized to attack one particular antigen (foreign proteins and carbohydrates) … See more As with many enzymes, RAG proteins are fairly large. For example, mouse RAG-1 contains 1040 amino acids and mouse RAG-2 contains 527 … See more It is still unclear what forces led to the development of a RAG1/2-mediated immune system exclusively in jawed vertebrates and not in any invertebrate species that also … See more • Sadofsky MJ (Aug 2004). "Recombination-activating gene proteins: more regulation, please". Immunological Reviews. 200: 83–9. doi:10.1111/j.0105-2896.2004.00164.x. PMID 15242398. S2CID 23905210. • De P, Rodgers KK (Aug 2004). "Putting the pieces … See more position von messi