WebH/NS condition protects pancreatic cancer cells from ferroptosis. (a) PANC1 and Patu8988 cells were treated with erastin (5 μM for PANC1 and 40 μM for Patu8988), RSL3 (3 μM for PANC1 or 5 μM for Patu8988), or sulfasalazine (2 mM) cultured in normal or H/NS condition for 24 hours.Cell viability was measured using a CCK8 kit. WebIn addition, AMPKα1/α2 knockdown PANC1 cells were resistant to erastin- or RSL3-induced cell death under high-glucose conditions but not under glucose starvation (Figure 4O). The average RNAi efficiency of target genes (AMPKα1/α2 or PDK4) in PANC1 and/or MEFs was greater than 90%.
GPX4 inhibitor induces autophagy a MIAPaCa2 and …
WebSLC2A1-dependent glucose uptake facilitates ferroptosis (A) Heatmap of cell viability in human PDAC cell lines (PANC1 and MIAPaCa2) or primary cells (pHsPDAC) following treatment with erastin... WebJan 28, 2024 · Pharmacological or genetic blockade of the NUPR1-LCN2 pathway (using NUPR1 shRNA, LCN2 shRNA, pancreas-specific Lcn2 conditional knockout mice, or the small molecule ZZW-115) increases the activity... crack poppy playtime
Current understanding of ferroptosis in the progression and …
WebJan 28, 2024 · Quantitative polymerase chain reaction (qPCR) confirmed that both erastin and RSL3 induced the upregulation of NUPR1 mRNA in four human PDAC cell lines … We would like to show you a description here but the site won’t allow us. WebApr 1, 2024 · (a) PANC1 and Patu8988 cells were treated with erastin (5 μM for PANC1 and 40 μM for Patu8988), RSL3 (3 μM for PANC1 or 5 μM for Patu8988), or sulfasalazine (2 mM) cultured in normal or... WebFeb 5, 2024 · ESCRT-III-dependent membrane repair blocks ferroptosis Ferroptosis is a form of regulated cell death that is triggered by iron accumulation and lipid peroxidation. Although plasma membrane injuries represent an important event in cell death, the impact of membrane repair mechanisms on ferroptosis remains unidentified. crack poster