Ctll 2 proliferation assay
WebCTLL-2 proliferation assay CTLL-2 cells were washed twice with prewarmed HBSS (Gibco, # 14175095) and then grown in the absence of IL-2 for 24 h in RPMI1640 (Gibco, … WebProliferation: IL-2 : CTLL-2 (ATCC TIB-214) 2: 24: 10: ≤175pg/mL: Proliferation: IL-3 : TF-1 (ATCC CRL-2003) 2: 48: 10: ≤0.6-0.15ng/mL: Proliferation: IL-4 : TF-1 (ATCC CRL-2003) 2: 48: 10: ... Cytotoxicity Assay: VEGF 121: HUVEC: 2: 72: 1000: ≤8-10ng/mL: Proliferation: Mouse Cytokine Bioassay Quick Guide Cytokine Indicator Cells Cell ...
Ctll 2 proliferation assay
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WebThe ED 50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10 7 IU/mg. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB, pH3.5, with 5 % ... WebWhen maintained in continuous culture, however, the overall sensitivity of this cell line is highly variable. To overcome this problem, large batches of CTLL-2 were grown, frozen and tested for their ability to detect low levels of IL-2 (0.01 IU) in dose response assays. Batches meeting this criterion were stored in liquid nitrogen until required.
WebFor example, Recombinant Human IL-2 can activate proliferation of IL-2 dependent cell lines, CTLL-2 and HT-2. The same lot of recombinant protein provides different ED 50 values depending on the cell line used for stimulation. In the CTLL-2 stimulation assay, the ED 50 is 0.26 ng/ml corresponding to a specific activity of 3.73 x10 6 units/mg. WebThe mouse T-cell line CTLL-2 is known to be dependent on interleukin-2 (IL-2) for both growth and viability. ... Although only very limited proliferation was observed during the incubation as assessed by 3H-thymidine incorporation, the viability of the CTLL-2 cells on the immobilized IL-2 matrix was preserved. Cells incubated on the immobilized ...
WebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents. Webinterleukin-15, antagonist, peptide, serum, CTLL-2 cell line, proliferation assay. ABSTRACT Dysregulated expression of Interleukin (IL)-15 has been associated to several autoimmune and inflammatory diseases. In this sense, some agents that inhibit IL-15 activity have been developed as possible drugs to treat these pathologies.
Webfrom the Mo7e and CTLL-2 cyto-kine-dependent proliferation assay, PFC-1 exhibited an approx-imately 10-fold stronger potency than rhIL-15 in PBMC prolifera-tion, while 10 nM rhIL-15 led to a proliferation rate of 20.71% and 1 nM PFC-1 resulted in a mean value of 22.05% (Fig. 2B). The enhanced activity could be due to the higher activity of IL ...
WebCellGenix® GMP rh IL-2 has an activity of 8 – 32 × 106 IU/mg. The activity of GMP rh IL-2 was measured in a cell proliferation assay using the IL-2-dependent cell line CTLL-2. It was calibrated against Proleukin®. You can find the batch specific activity on the certificate of analysis (CoA). shaq and his new girlfriendWebNov 29, 2013 · Most bioassays measured the proliferative effect of IL-2 on the murine cytotoxic T cell-line, CTLL-2 or the HT-2 cell-line but employed different readouts for assessing the proliferation (Gillis et al., 1978, Gieni et al., 1995). In one laboratory, however, the human T cell-line, KIT 225 was used (Hori et al., 1987). pooh up down touch the ground tik tokWebMay 24, 2015 · Cell Proliferation Assay Application of a colorimetric CTLL-2 cell proliferation assay for the evaluation of IL-15 antagonists Authors: Yunier Rodríguez Center for Genetic Engineering and... pooh waffle ironWebApr 14, 2024 · Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively). 6'6 3$*(5HFRPELQDQW+XPDQ,/ KÉ 3URWHLQ6'6 3$*( 2 μg/lane of Recombinant Human ILKÉ2 Protein (Catalog # BT-002) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and … shaq and his wife imageshttp://www.wrenshallab.com/uploads/5/6/3/8/56385051/mttctll2.pdf pooh vhs archive.orgWebAug 31, 2009 · A colorimetric cell proliferation assay using soluble tetrazolium salt [(CellTiter 96(R) Aqueous One Solution) cell proliferation reagent, containing the (3-(4,5 … pooh\u0027s super sleuth christmas movieWebApr 11, 2024 · Recombinant Mouse IL2 (Catalog # 402 ML) stimulates cell proliferation of the CTLL 2 mouse cytotoxic T cell line. The ED 50 for this effect is 0.1 0.4 ng/mL. 6'6 3$*(1 μg/lane of Recombinant Mouse IL2 was resolved with SDSPAGE under reducing (R) conditions and visualized by silver staining, shaq and hot chip challenge